| Nervous system nuclear protein induced by axotomy 1 (Nna1, AGTPBP1 or cytosolic carboxypeptidase-1) is related to zinc carboxypeptidases and contains an ATP/GTP binding motif, a basic and a bipartite nuclear localization signal. Nna1 expression is rapidly induced |
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| in motor neurons following axotomy and down-regulated following reinnervation, which is consistent with abundant Nna1 expression in differentiating neurons and the absence |
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| of Nna1 in CNS proliferative zones (1). Furthermore, Purkinje cell degeneration is characterized by adult onset neurodegeneration resulting from Nna1 gene mutations, implicating Nna1 in mechanisms common to degeneration and regeneration (2). |
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| Catalog # | N2920 |
| Applications | Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. |
| Recommended Dilution | Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, |
| 0.1% Tween-20 at 4°C with gentle shaking, overnight. |
| Immunoprecipitation: 1:50 |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | As reported |
| Form | Supplied as a liquid in 10mM sodium HEPES pH 7.5, 0.1%, 150mM NaCl, 100ug/ml BSA and 50% glycerol. |
| Purity | Purified by Protein A affinity chromatography. |
| Immunogen | Synthetic peptide corresponding to residues surrounding Asp1175 of human Nna1 protein (KLH-coupled). |
| Specificity | Recongnizes endog-enous levels of human total Nna1 protein. Species crossreactivity: mouse, rat. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
