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You are here:Home » Antibodies » Antibodies-Nitric Oxide » Anti -Nitroso-L-Histidine (NO-Histidine), Conjugated

Anti -Nitroso-L-Histidine (NO-Histidine), Conjugated


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Clone Host Grade Applications
Polyclonal Rat Purified E
Catalog #N2940-10
Applications Suitable for use in ELISA. Other applications not tested.
Specificity Using a conjugate NO-Histidine-Glutaraldehyde-Protein, antibody specificity was performed with an ELISA test by competition experiments with the following compounds.
Compound Cross-reactivity ratio (a) NO-Histidine-G-BSA 1 Histidine-G-BSA 1/>100 000 NO-Cysteine-G-BSA 1/>100 000 NO-5HT-G-BSA 1/>100 000 NO-Tryptophan-G-BSA 1/65 NO-Tyrosine-G-BSA 1/307
(a) NO-Histidine-G-BSA concentration/unconjugated or conjugated close-related compounds concentration at half displacement.
G = Glutaraldehyde, BSA = Bovine Serum Albumin
Recommended dilution The anti-conjugated NO-Histidine antibodies can be diluted between 1/2,000-1/5,000.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
FormSupplied as a liquid in PBS, pH 7.2. No preservative added.
PurityAntiserum previously preabsobed on protein carriers, and purified by ammonium sulfate precipitation and gel filtration.
SpecificityRecognizes protein-conjugated NO-L-Histidine. See additional crossreactivity data.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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