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Anti -Nitroso-L-Methionine (NO-L-Methionine), Conjugated
Anti -Nitroso-L-Methionine (NO-L-Methionine), Conjugated
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rat |
Purified |
E |
|
| Catalog # | N2940-55 | | Applications | Suitable for use in ELISA. Other applications not tested. | | Specificity Using a conjugate NO-Methionine-Glutaraldehyde-Protein, antibody specificity was performed with an ELISA test by competition experiments with the following compounds. | | Compound Cross-reactivity ratio (a) NO-Methionine-G-BSA 1 Methionine-G-BSA 1/2,500 NO-Cysteine-G-BSA 1/>50,000 NO2-Tyrosine-G-BSA 1/6,000 | | (a) | NO-Methionine-G-BSA concentration/unconjugated or conjugated close-related compounds concentration at half displacement. | | G = Glutaraldehyde, BSA = Bovine Serum Albumin | | Recommended dilution The anti-conjugated NO-Methionine antibodies can be diluted between 1/2,000-1/5,000. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rat | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.2. No preservative added. | | Purity | Antiserum previously preabsobed on protein carriers, and purified by ammonium sulfate precipitation and gel filtration. | | Immunogen | NO-Methionine-Glutaraldehyde-BSA | | Specificity | Recognizes protein-conjugated NO-L-Methionine. See additional crossreactivity data. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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