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You are here:Home » Antibodies » Abs to Nitric Oxide » Anti -Nitroserotonin (NO2-Serotonin), Conjugated

Anti -Nitroserotonin (NO2-Serotonin), Conjugated

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Purified E
Catalog #N2940-90
Applications
Suitable for use in ELISA. Other applications not tested.
SpecificityUsing a conjugate NO2-Serotonin-Glutaraldehyde-BSA, antibody specificity was performed with an ELISA test by competition experiments with the following compounds:
Compound Cross-reactivity ratio NO2-5HT-G-BSA 1 5HT-G-BSA 1100,000
NO2-5HT 1:100,000
NO-5HT-G-BSA 1525
NO-Cys-G-BSA 1:100,000
NO-W-G-BSA 1:100,000
NO-His-G-BSA 1:100,000
NO2-Serotonin-G-BSA concentration/unconjugated or conjugated close-related compounds concentration at half displacement; G = Glutaraldehyde
Recommended DilutionThe anti-conjugated NO2-Serotonine antibodies can be diluted between 1/2,000-1/5,000.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2. No preservative added.
PurityAntiserum previously preabsobed on protein carriers, and purified by ammonium sulfate precipitation and gel filtration.
ImmunogenNO2-Serotonin-Glutaraldehyde-BSA
SpecificityRecognizes protein-conjugated NO2-Serotonin. See additional crossreactivity data.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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