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You are here:Home » Antibodies » Abs to Nitric Oxide » Anti -nNOS

Anti -nNOS

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH
Nitric oxide (NO) is a potent bio-regulatory molecule, which plays important roles in the regulation of a variety of normal developmental and physiological processes. NO is generated by nitric oxide synthase (NOS) in a reaction which converts L-arginine and oxygen into citrulline and nitric oxide. Three distinct mammalian genes have been identified which encode NOS isoforms: neuronal (nNOS or bNOS), macrophage or inducible (iNOS), and endothelial (eNOS). The NOS isoforms can be subdivided into two general categories, constitutive or inducible, based on differences in their regulation and activities. The constitutive isoforms include eNOS and nNOS.These isoforms are always present but remain inactive until an increase in intracellular calcium results in enhanced calcium/calmodulin binding and subsequent activation. In contrast, the inducible NOS isoform, iNOS, is not normally expressed, but is induced (though alterations in gene expression, mRNA stability, or protein synthesis) in response to certain cytokines.
Catalog #N2950-07G
ApplicationsSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsELISA: 0.1-1.0ug/ml
Western Blot: 1ug/ml
Immunohistochemistry (Paraffin): 5-10ug/ml. Some background staining is evident in paraffin embedded tissues
Immunohistochemistry (Frozen): 1-2ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceRat
Concentration~0.25mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenA recombinant protein consisting of 195 amino acids from the N-terminal of rat nNOS protein.
SpecificityRecognizes the ~160kD nNOS protein and does not exhibit any crossreactivity with the related eNOS or iNOS proteins.
Species CrossreactivityReacts with a ~160kD band on Western Blots of rat and mouse brain tissue lysates (20ug).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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