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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B IH

Nitric oxide (NO) is a potent bio-regulatory molecule, which plays important roles in the regulation of a variety of normal developmental and physiological processes. NO is generated by nitric oxide synthase (NOS) in a reaction which converts L-arginine and oxygen into citrulline and nitric oxide. Three distinct mammalian genes have been identified which encode NOS isoforms: neuronal (nNOS or bNOS), macrophage or inducible (iNOS), and endothelial (eNOS). The NOS isoforms can be subdivided into two general categories, constitutive or inducible, based on differences in their regulation and activities. The constitutive isoforms include eNOS and nNOS.These isoforms are always present but remain inactive until an increase in intracellular calcium results in enhanced calcium/calmodulin binding and subsequent activation. In contrast, the inducible NOS isoform, iNOS, is not normally expressed, but is induced (though alterations in gene expression, mRNA stability, or protein synthesis) in response to certain cytokines.
Catalog #	N2950-07G
Applications	Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions	ELISA: 0.1-1.0ug/ml
	Western Blot: 1ug/ml
	Immunohistochemistry (Paraffin): 5-10ug/ml. Some background staining is evident in paraffin embedded tissues
	Immunohistochemistry (Frozen): 1-2ug/ml
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Rat
Concentration	~0.25mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Purity	Purified by immunoaffinity chromatography.
Immunogen	A recombinant protein consisting of 195 amino acids from the N-terminal of rat nNOS protein.
Specificity	Recognizes the ~160kD nNOS protein and does not exhibit any crossreactivity with the related eNOS or iNOS proteins.
Species Crossreactivity	Reacts with a ~160kD band on Western blots of rat and mouse brain tissue lysates (20ug).
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.