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You are here:Home » Cloning » Cloning-Primers » NOS primers, Hu endothelial, amplimer 485bp

NOS primers, Hu endothelial, amplimer 485bp

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Specifications

Catalog #N2991
ContentsPCR primers for use in the detection and analysis of human mRNA transcript. This PCR-Amplimer set amplifies a specific gene transcript of size 485bp. The amplimer set includes 5' and 3' primers in separate tubes compatible with regular PCR reagents. Each set (each primer in a 50ul volume) is sufficient for at least 100 reactions.
Primer A Sense Primer
Primer B: Antisense Primer
Storage and Stability-20°C in a constant-temperature freezer.
Optimal PCR ConditionsNumber of cycles: 30
Denaturation temperature: 96°C for 35 secs.
Annealing temperature: 62°C for 2 min.
Extension temperature: 72°C for 2 min. in 1mM MgCl2.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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