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You are here:Home » Antibodies » Abs to Neutrophils » Anti -Neutrophils, Apoptotic

Anti -Neutrophils, Apoptotic

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified IP IH IC FC
Neutrophils are recruited in large numbers to inflammation sites. Clearance of these cells from the inflammatory site is done by programmed cell death (apoptosis) of the neutrophil. As a consequence, many cellular mechanisms are down-regulated in the apoptotic neutrophil such as: adhesion, chemotaxis, phagocytosis, enzyme secretion, generation of a respiratory burst, and responsiveness to external stimuli. Changes in the cell surface of dying neutrophils are recognized by macrophages, which destroy the neutrophils by phagocytosis. Many changes in the amount of proteins, carbohydrates and lipids on the surface of neutrophils are associated with the apoptotic event. Apoptotic Neutrophils (BOB78) is an up-regulated protein, molecular weight 90 kDa, that appears in the granular compartment of the late apoptotic neutrophil. This antigen does not appear in freshly isolated erythrocytes, platelets, lymphocytes, monocytes, or neutrophils. Monoclonal antibodies specific for BOB78 are a useful tool for studying the biology of apoptotic neutrophils.
Catalog #N5201
Applications
Suitable for use in Flow Cytometry, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionFlow Cytometry: 2ug/ml
Optimal dilutions to be determined by the researcher.
Positive ControlTHP-1 cells
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgM,k
Clone No10H1
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1M Tris-Glycine, 150mM sodium chloride, 0.05% sodium azide.
PurityPurified by ammonium sulfate precipitation.
ImmunogenHuman whole apoptotic neutrophils BOB78.
SpecificityRecognizes human Apoptotic Neutrophils.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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