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You are here:Home » Antibodies » Abs to Nitric Oxide » Anti -NOS (uNOS, Universal Nitric Oxide Synthase)

Anti -NOS (uNOS, Universal Nitric Oxide Synthase)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites B IH
Catalog #N5349-08
ApplicationsSuitable for use in Immunohistochemistry and Western Blot. Other applications not tested.
Recommended DilutionImmunohistochemistry (frozen sections): 1:100
Western Blot: 1:250
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgM
Clone NoNOS-3F7-B11-B5
HostMouse
SourceBovine
ConcentrationNot determined
FormLiquid. Ascites diluted in PBS containing 0.05% sodium azide.
PurityAscites
ImmunogenPurified neuronal nitric oxide synthase (nNOS; NOS I) from bovine brain.
SpecificityRecognizes iNOS (NOS II), eNOS (NOS III) and nNOS (NOS I) in mouse, rat and bovine samples. Detects bands of ~130kDa (representing iNOS in samples first induced with interferon (IFN) and lipopolysaccarides (LPS)), ~155kDa (in tissues expressing nNOS) and ~140kDa (in tissues expressing eNOS) by Western blot.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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