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You are here:Home » Antibodies » Abs to Nitric Oxide » Anti -NOS (uNOS, Universal Nitric Oxide Synthase)

Anti -NOS (uNOS, Universal Nitric Oxide Synthase)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
Catalog #N5349-08C
ApplicationsSuitable for use in Western Blot and Immunohistochemistry (paraffin-embedded sections). Other applications not tested.
Recommended DilutionImmunohistochemistry: 1:50 using paraffin sections. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.
Cellular Localization Cytoplasmic
Positive Control HUVEC cells and placenta cells.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
Concentration~0.2mg/ml
FormSupplied as a liquid in PBS. pH 7.4, with BSA and sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to amino acids located within the C-terminal region of mouse NOS
SpecificityThis antibody reacts with iNOS, bNOS and eNOS. Species Crossreactivity: human, mouse, rat, bovine and porcine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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