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You are here:Home » Antibodies » Abs to Nitric Oxide » Anti -NOS I (nNOS, bNOS, Nitric Oxide Synthase, NOS 1)

Anti -NOS I (nNOS, bNOS, Nitric Oxide Synthase, NOS 1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH N
Immunohistochemistry: Use at 510ug/ml on rat neuronal sections fixed with 3.5% paraformaldehyde. Incubation overnight at 4°C with a 1:1001:500 dilution. ABC (peroxidase) detection. Positive staining of Purkinje cells and Layer 5 primary neurons. Western blot: 110ug/ml with chemiluminescent detection. Higher concentrations of the antibody may be necessary if colorimetric detection is used. Antibody dilution will also vary with the concentration of antigen. Then antibody should recognize a 155kD protein. ELISA: Use at 1.0ug/ml or lower on plates coated with 50100ng of peptide antigen.
Catalog #N5350-01
Clone TypePolyclonal
HostRabbit
SourceHuman
Concentration~1ug/ul
FormSupplied as a liquid in PBS, pH 7.4, containing 0.1% BSA and 0.1% sodium azide.
PurityAffinity Purified
ImmunogenSynthetic peptide corresponding to amino acids 724739 of human brain NOS, coupled to KLH.
SpecificityReactive with human brain NOS-I (nNOS). Antibody binding is neutralized in Western blot by pre-adsorption with the immunogen peptide. This sequence is highly conserved in human, rat and mouse.
Important NoteThis product is intended for research use only, not for use in human, therapeutic or diagnostic applications.


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