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You are here:Home » Antibodies » Abs to Nitric Oxide » Anti -NOS I (nNOS, bNOS, Nitric Oxide Synthase, NOS 1)

Anti -NOS I (nNOS, bNOS, Nitric Oxide Synthase, NOS 1)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites
Nitric oxide (NO), a diffusible free radical gas, acts as a neurotransmitter in brain and peripheral nervous system.  It accounts for the activity of endothelium-derived relaxing factors, which stimulate vasodilatation by releasing NO from the endothelium. Unlike typical neurotransmitter, NO is a not stored in synaptic vesicle and does not act on membrane receptors.  Synthesis of NO, initially demonstrated in vascular endothelium, is now found in many tissues.
NO is synthesized by L-arginine, oxygen, and NADPH by three known isoforms of heme-containing flavoproteins termed NO synthase (NOS, I-III, mol wt. ~130-160kD).  One group of enzyme is constitutive, agonist-triggered, and dependent on Ca2+/Calmodulin and is inhibited by L-arginine analogues (L-NNA, L-NMMA).  It is found in endothelium, adrenal glands, brain and platelets.  The other principle group is inducible, Ca2+/Calmodulin-independent, and inhibited by NMMA and L-NNA.  It has been found in macrophage, hepatocytes, tumor cells, vascular smooth muscle and endothelial cells.  Analyses of cDNA clones have identified three distinct NOS genes in mammals: neuronal (nNOS/bNOS/NOS-I), endothelial (eNOS/NOS-III), and macrophage (mNOS/iNOS/NOS-II).  Both nNOS and eNOS are constitutive and the mNOS/iNOS is inducible.  Sequence homology among different cloned isoforms is ~ 50%.
Catalog #N5350-01G
ApplicationsSuitable for use in Western Blot and ELISA. Other applications not tested.
Recommended DilutionWestern Blot: 1:1K or more before use. The antibody recognizes 130kD protein in Western Blots.
ELISA: Control anigen should be coated at 1ug/ml.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No7C40
HostMouse
SourceRat
ConcentrationAs reported
FormSupplied as a lyophilized powder from PBS, 0.05% sodium azide.
PurityAscites
ImmunogenAmino terminal region (aa1-180) of rat brain NOS I, expressed as fusion protein
SpecificityRecognizes rat NOS I. Species Crossreactivity: Mouse and human. No significant reactivity is seen with NOS-2/NOS-3.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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