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You are here:Home » Antibodies » Abs to Nitric Oxide » Anti -NOS I (Nitric Oxide Synthase 1 (neuronal), NOS type I, NOS1, NOS 1, Constitutive NOS, IHPS1, Neuronal Nitric Oxide Synthase, Neuronal NOS, nNOS, N-NOS, NC-NOS, Nitric Oxide Synthase Brain, bNOS, NO, NOS)

Anti -NOS I (Nitric Oxide Synthase 1 (neuronal), NOS type I, NOS1, NOS 1, Constitutive
NOS, IHPS1, Neuronal Nitric Oxide Synthase, Neuronal NOS, nNOS, N-NOS, NC-NOS, Nitric
Oxide Synthase Brain, bNOS, NO, NOS)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
Three isoforms of nitric oxide synthase (NOS) have been identified. All are homodimers with subunits of 130-160kD. All have binding sites for NADPH, FAD, and FMN near the carboxyl terminus (the reductase domain), and binding sites for tetrahydrobiopterin (BH4) and heme near the amino terminus (the oxygenase domain). The reductase and oxygenase domains are linked by a calmodulin (CaM) binding site. Occupation of this site facilitates electron transfer from the cofactors in the reductase domain to heme during nitric oxide production. NOS catalyzes the conversion of arginine to citrulline and nitric oxide (NO). Neuronal nitric oxide synthase (nNOS, bNOS, cNOS, Type I) is associated with the post-synaptic density protein (PSD-95) in the neuronal membrane. In response to increased intracellular Ca2+, nNOS interacts with CaM. The Ca2+-CaM complex, in combination with BH4, binds to nNOS and induces its translocation from the plasma membrane to the cytoplasm. The dephosphorylation of nNOS by calcineurin initiates the production NO. NO activates guanylyl cyclase (GC) and activates the various cGMP-regulated signaling pathways. nNOS is in activated by phosphorylation by protein kinase A (PKA) or protein kinase C (PKC).
Catalog #N5350-01M
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in TBS, pH 7.2, 0.5% BSA.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide with sequence ESKKDTDEVFSS, representing the C-terminus of the human protein (residues 1423-1434) according to NP_000611.
SpecificitySpecies Crossreactivity: Mouse. Expected to crossreact with Human, canine, and Rabbit due to sequence homology.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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