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You are here:Home » Antibodies » Abs to Nitric Oxide » Anti -NOS1 (Nitric Oxide Synthase, Brain, Constitutive NOS, NC-NOS, NOS Type I, Neuronal NOS, N-NOS, nNOS, bNOS)

Anti -NOS1 (Nitric Oxide Synthase, Brain, Constitutive NOS, NC-NOS, NOS Type I, Neuronal
NOS, N-NOS, nNOS, bNOS)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B
Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains. bNOS and eNOS share approximately 50% sequence homology and their enzymatic activity depends on binding to the calcium/calmodulin complex. Increases in intracellular calcium lead to the production of low levels of NO over a short time. bNOS is found in neurons, peripheral nerve cells, macula densa, and pancreatic islet cells. Alternate splicing specifically regulates bNOS in striated muscle. The translated protein, bNOS mu, is 34aa residues larger than bNOS from brain.
Catalog #N5350-01S
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 0.5ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with 1.2% sodium acetate. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No10K295
HostMouse
ConcentrationAs reported
FormSupplied as a lyophilized powder from 1.2% sodium acetate, 2mg BSA, 0.01mg sodium azide. Reconstitute with 1.2% sodium acetate, PBS.
PurityPurified by immunoaffinity chromatography.
ImmunogenProtein A fusion protein.
SpecificityRecognizes NOS1. Species crossreactivity: human, rat, goat, porcine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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