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You are here:Home » Antibodies » Abs to Nitric Oxide » Anti -NOS I, phosphorylated (Ser1417) (Neuronal Nitric Oxide Synthase, bNOS, nNOS, NOS-1)

Anti -NOS I, phosphorylated (Ser1417) (Neuronal Nitric Oxide Synthase, bNOS, nNOS, NOS-1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Nitric oxide (NO), a diffusible free radical gas, acts as a neurotransmitter in brain and peripheral nervous system. It accounts for the activity of endothelium-derived relaxing factors, which stimulate vasodilatation by releasing NO from the endothelium. Unlike typical neurotransmitter, NO is a not stored in synaptic vesicle and does not act on membrane receptors. Synthesis of NO, initially demonstrated in vascular endothelium, is now found in many tissues. It is found in endothelium, adrenal glands, brain and platelets. The other principle group is inducible, Ca2+/Calmodulin-independent, and inhibited by NMMA and L-NNA. It has been found in macrophage, hepatocytes, tumor cells, vascular smooth muscle and endothelial cells. Analyses of cDNA clones have identified three distinct NOS genes in mammals: neuronal (nNOS/bNOS/NOS-I), endothelial (eNOS/NOS-III), and macrophage (mNOS/iNOS/NOS-II).
Catalog #N5350-02
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:500 detects affinity purified bacterial expressed rat nNOS/NOS I that had been phosphorylated by purified AMPK in vitro. Lambda phosphatase treated and untreated affinity purified bacterial expressed rat nNOS/NOS I that had been phosphorylated by purified AMPK was resolved by electrophoresis, transferred to PVDF and probed with N5350-02 (1:500). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies I1904-46E IgG, H&L (HRP) Dk xRb
I1904-46K: IgG, H&L, X-Adsorbed (HRP) Dk xRb
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.6, 0.175M sodium chloride, 0.07% sodium azide, before the addition of glycerol to 30%.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to aa surrounding Ser1417 of human neuronal Nitric Oxide Synthase, with an N-terminal cysteine added for KLH conjugation purposes, where pS is phosphorylated serine corresponding to the AMPK phosphorylation site.
SpecificityRecognizes human phosphorylated nNOS/NOS I, Mr 170kD. Species Cross-reactivity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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