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You are here:Home » Antibodies » Abs to Nitric Oxide » Anti -NOS I (Nitric Oxide Synthase I, bNOS, nNOS, NOS-I)

Anti -NOS I (Nitric Oxide Synthase I, bNOS, nNOS, NOS-I)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum B IC
Catalog #N5350-09
ApplicationsSuitable for use in Western Blot and Immunocytochemistry. Other applications not tested.
Recommended DilutionImmunoblot Analysis: A 1:4000-1:10,000 dilution of this lot detected nNOS (Mr ~165kD) in a rat brain microsomal preparation. The use of fresh extract preparations is recommended.
Immunocytochemistry: Has been reported by an independent laboratory to detect nNOS in C2C12 myotubes.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceRat
ConcentrationNot determined
FormSupplied as a liquid with 0.05% sodium azide.
PuritySerum
ImmunogenSynthetic peptide corresponding to N-terminus of rat nNOS (neuronal nitric oxide synthase)
SpecificityRecognizes nNOS, Mr 165kD. Species Crossreactivity: Human, mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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