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You are here:Home » Antibodies » Abs to Nitric Oxide » Anti -Nos2 (Nitric Oxide Synthase, Inducible, Inducible NO Synthase, Inducible NOS, iNOS, Macrophage NOS, MAC-NOS, NOS Type II, Peptidyl-cysteine S-nitrosylase NOS2, Inosl)

Anti -Nos2 (Nitric Oxide Synthase, Inducible, Inducible NO Synthase, Inducible NOS, iNOS,
Macrophage NOS, MAC-NOS, NOS Type II, Peptidyl-cysteine S-nitrosylase NOS2, Inosl)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
Catalog #N5350-10F
NOS oxidizes a guanidine nitrogen of arginine releasing nitric oxide in the form of a free radical and citrulline. Notric oxide generated, acts as a messenger in diverse functions including vasodilation neurotransmission, anti-tumor and anti-pathogenic activities. Inducible NOS (iNOS) or macrophage NOS (mNOS) is a calcium/calmodulin independent and expressed in activated macrophages and stimulated glial cells.
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1-2ug/ml for 2h, RT
Immunohistochemistry: 1:100 for 10min at RT
Staining of formalin-fixed tissues requires boiling tissues sections in 10mM citrate buffer, pH 6.0, for 10min followed by cooling at RT for 20min
Optimal dilutions to be determined by the researcher.
Positive ControlLung
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
HostRabbit
SourceMouse
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to the C-terminus of mouse iNOS.
SpecificityRecognizes mouse iNOS. Species Crossreactivity: human and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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