Anti -Nos2 (Nitric Oxide Synthase, Inducible, Inducible NO Synthase, Inducible NOS, iNOS,
Macrophage NOS, MAC-NOS, NOS Type II, Peptidyl-cysteine S-nitrosylase NOS2, Inosl)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
B IH |
|
| Catalog # | N5350-10F | | NOS oxidizes a guanidine nitrogen of arginine releasing nitric oxide in the form of a free radical and citrulline. Notric oxide generated, acts as a messenger in diverse functions including | vasodilation neurotransmission, anti-tumor and anti-pathogenic activities. Inducible NOS (iNOS) or macrophage NOS (mNOS) is a calcium/calmodulin independent and expressed in activated macrophages and stimulated glial cells. | | Applications | Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Western Blot: 1-2ug/ml for 2h, RT | | Immunohistochemistry: 1:100 for 10min at RT | | Staining of formalin-fixed tissues requires boiling tissues sections in 10mM citrate buffer, pH 6.0, for 10min followed by cooling at RT for 20min | | Optimal dilutions to be determined by the researcher. | | Positive Control | Lung | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Host | Rabbit | | Source | Mouse | | Concentration | ~0.1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Synthetic peptide corresponding to the C-terminus of mouse iNOS. | | Specificity | Recognizes mouse iNOS. Species Crossreactivity: human and rat. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
External Links
|
