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You are here:Home » Antibodies » Abs to Nitric Oxide » Anti -NOS3 (Nitric Oxide Synthase, Endothelial, Constitutive NOS, cNOS, EC-NOS, Endothelial NOS, eNOS, NOS Type III, NOSIII)

Anti -NOS3 (Nitric Oxide Synthase, Endothelial, Constitutive NOS, cNOS, EC-NOS,
Endothelial NOS, eNOS, NOS Type III, NOSIII)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites B IP IH
Endothelial nitric-oxide synthase (eNOS) is an important enzyme in the cardiovascular system. It catalyzes the production of nitric oxide (NO), a key regulator of blood pressure, vascular remodeling, and angiogenesis (1,2). The activity of eNOS is regulated by phosphorylation at multiple sites. The two most thoroughly studied sites are the activation site Ser1177 and the inhibitory site Thr495 (3). Several protein kinases including Akt/PKB, PKA, and AMPK activate eNOS by phosphorylating Ser1177 in response to various stimuli (4,5). In contrast, bradykinin and H2O2 activate eNOS activity by promoting both Ser1177 phosphorylation and Thr495 dephosphorylation (6,7).
Catalog #N5350-20N
ApplicationsSuitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000
Immunohistochemistry (paraffin): 1:100
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No6H2
HostMouse
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, <0.02% sodium azide, 50% glycerol.
PurityAscites
ImmunogenRecombinant corresponding to human eNOS.
SpecificityRecognizes endogenous levels of total human eNos. Species Crossreactivity: bovine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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