Anti -NOS III, CT (Nitric Oxide Synthase 3, Nitric Oxide Synthase 3 Endothelial Cell, NOS Type III, NOS3, Endothelial Nitric Oxide Synthase 3, Endothelial NOS, eNOS, ECNOS, EC-NOS, Constitutive NOS, cNOS, Nitric Oxide Synthase Endothelial)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
B |
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| Endothelial NOS (eNOS), also known as Nitric oxide synthase 3 (NOS3) or constitutive NOS (cNOS), is a nitric oxide synthase. It generates NO in blood vessels and is involved with regulating vascular function. A constitutive Ca2+ dependent NOS provides a basal release of NO. eNOS is associated with plasma membranes surrounding cells and the membranes of Golgi bodies within cells. | | | Catalog # | N5350-22 | | Applications | Suitable for use in Western Blot. Other applications not tested. | | Recommended Dilution | Western Blot: 0.5-2ug/ml detects eNOS/NOS III in RIPA lysates from HUVEC cells. | | Optimal dilutions to be determined by the researcher. | | Recommended Control | Platelets, placenta, liver, kidney | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.6, 0.07% sodium azide, before the addition of glycerol to 30%. | | Purity | Purified by Protein A affinity chromatography. | | Immunogen | Synthetic peptide corresponding to aa1181-1194, C-QERQLRGAVPWAFD of human eNOS/NOS III, KLH conjugated. | | Species Sequence Homology | Mouse, guinea pig, porcine, rat and canine; 10/11aa. | | Specificity | Recognizes human eNOS/NOS III, Mr ~135kD. Species Crossreactivity: bovine. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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Download Specifications for Anti -NOS III, CT (Nitric Oxide Synthase 3, Nitric Oxide Synthase 3 Endothelial Cell, NOS Type III, NOS3, Endothelial Nitric Oxide Synthase 3, Endothelial NOS, eNOS, ECNOS, EC-NOS, Constitutive NOS, cNOS, Nitric Oxide Synthase Endothelial) |
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