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You are here:Home » Antibodies » Abs to Nitric Oxide » Anti -NOS III, CT (Nitric Oxide Synthase 3, Nitric Oxide Synthase 3 Endothelial Cell, NOS Type III, NOS3, Endothelial Nitric Oxide Synthase 3, Endothelial NOS, eNOS, ECNOS, EC-NOS, Constitutive NOS, cNOS, Nitric Oxide Synthase Endothelial)

Anti -NOS III, CT (Nitric Oxide Synthase 3, Nitric Oxide Synthase 3 Endothelial Cell, NOS
Type III, NOS3, Endothelial Nitric Oxide Synthase 3, Endothelial NOS, eNOS, ECNOS, EC-NOS,
Constitutive NOS, cNOS, Nitric Oxide Synthase Endothelial)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Endothelial NOS (eNOS), also known as Nitric oxide synthase 3 (NOS3) or constitutive NOS (cNOS), is a nitric oxide synthase. It generates NO in blood vessels and is involved with regulating vascular function. A constitutive Ca2+ dependent NOS provides a basal release of NO. eNOS is associated with plasma membranes surrounding cells and the membranes of Golgi bodies within cells.
Catalog #N5350-22
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 0.5-2ug/ml detects eNOS/NOS III in RIPA lysates from HUVEC cells.
Optimal dilutions to be determined by the researcher.
Recommended ControlPlatelets, placenta, liver, kidney
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.6, 0.07% sodium azide, before the addition of glycerol to 30%.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide corresponding to aa1181-1194, C-QERQLRGAVPWAFD of human eNOS/NOS III, KLH conjugated.
Species Sequence HomologyMouse, guinea pig, porcine, rat and canine; 10/11aa.
SpecificityRecognizes human eNOS/NOS III, Mr ~135kD. Species Crossreactivity: bovine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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