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You are here:Home » Antibodies » Abs to Nitric Oxide » Anti -NOS III (Nitric Oxide Synthase 3, Nitric Oxide Synthase 3 Endothelial Cell, NOS Type III, NOS3, Endothelial Nitric Oxide Synthase 3, Endothelial NOS, eNOS, ECNOS, EC-NOS, Constitutive NOS, cNOS, Nitric Oxide Synthase Endothelial)

Anti -NOS III (Nitric Oxide Synthase 3, Nitric Oxide Synthase 3 Endothelial Cell, NOS Type
III, NOS3, Endothelial Nitric Oxide Synthase 3, Endothelial NOS, eNOS, ECNOS, EC-NOS,
Constitutive NOS, cNOS, Nitric Oxide Synthase Endothelial)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum B IC
NOS oxidizes a guanidine nitrogen of arginine releasing nitric oxide in the form of a free radical and citrulline. Nitric oxide thus generated acts as a messenger in diverse functions including vasodilation neurotransmission, anti-tumor and antipathogenic activities.. NOS is classified under three types: Type I NOS is 150-160kD and is found in neurons. It is constitutively expressed and is Ca2+/calmodulin dependent. It is called NOS-1, neuronal NOS (nNOS), brain NOS (bNOS), cerebral / constitutive / Ca2+regulated NOS (cNOS). bOS is present in skeletal muscles and its absence in Duchenne’s muscular dystrophy accounts for its symptoms of the disease. Type II NOS is 130kD protein known as macrophage NOS (mNOS) or inducible NOS (iNOS). It is Ca2+/calmodulin independent and is expressed in activated macrophages and stimulated glial cells. Type III NOS is a 135kD protein called endothelial NOS (eNOS).
Catalog #N5350-23
ApplicationsSuitable for use in Western Blot and Immunocytochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 1:1000. Detects a band of ~140kD.
Immunocytochemistry: 1:250
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
ConcentrationNot Determined
FormSupplied as a liquid in PBS, 0.05% sodium azide.
PuritySerum
ImmunogenSynthetic peptide corresponding to aa598-612 P598YNSSPRPEQHKSYK612C of bovine endothelial nitric oxide synthase
SpecificityRecognizes eNOS (NOS III). Does not crossreact with inducible NOS (iNOS; NOS II) or neuronal NOS (nNOS; NOS I).
Species CrossreactivityHuman, mouse, rat and canine
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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