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You are here:Home » Antibodies » Abs to Nitric Oxide » Anti -NOS III, phosphorylated (Ser1177) (Endothelial Nitric Oxide Synthase, eNOS, NOS 3)

Anti -NOS III, phosphorylated (Ser1177) (Endothelial Nitric Oxide Synthase, eNOS, NOS 3)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Endothelial nitric-oxide synthase (eNOS) is an important enzyme in the cardiovascular system. It catalyzes the production of nitric oxide (NO), a key regulator of blood pressure, vascular remodeling and angiogenesis (1,2). eNOS is regulated by phosphorylation at multiple sites. The two most throughly studied sites are the activation site serine 1177 and the inhibitory site threonine 495 (3). Several protein kinases including Akt/PKB, PKA and AMPK activate eNOS by phosphorylating Ser1177 in response to various stimuli (4,5). In contrast, bradykinin and hydrogen peroxide activate eNOS activity by promoting Thr495 dephosphorylation (6,7).
Catalog #N5350-26
Recommended Dilution Western Blotting: 1:1000
Optimal dilutions to be determined by the researcher.
Storage and Stability May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Ser1177 of human eNOS.
SpecificityDetects endogenous levels of eNOS only when phosphorylated at Ser1177.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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