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Anti -NOS III, phosphorylated (Ser1177) (Endothelial Nitric Oxide Synthase, eNOS, NOS 3)
Anti -NOS III, phosphorylated (Ser1177) (Endothelial Nitric Oxide Synthase, eNOS, NOS 3)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Purified |
E B |
|
| Endothelial NO synthase produces nitric axide (NO) which plays a crucial role in cardiovascular homeostasis. Activation of the enzyme and NO production can be induced by phosphorylation of eNOS at serine 1177 by activated Akt/PKB. | | | Catalog # | N5350-26B | | Applications | | | Suitable for use in ELISA and Western Blot. Other applications have not been tested. | | Recommended Dilutions | | | Western Blot: 2ug/ml (HRP/ECL) | | ELISA: 0.1ug/ml | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1,k | | Clone No | 6D618 | | Host | Mouse | | Source | Human | | Concentration | ~0.1mg/ml | | Form | Supplied as a liquid in PBS, 0.09% sodium azide, PEG, sucarose, 50% glycerol. | | Purity | Purified by size exclusion chromatography. | | Immunogen | Synthetic peptide corresponding to a portion of human eNOS (endothelial nitric oxide synthase) phosphorylated at Ser1177. | | | Specificity | Recognizes human eNOS phosphorylated at Ser1177. Does not cross-react with non-phosphorylated eNOS or other unrelated serine-phosphorylated proteins. Species crossreactivity: mouse. | | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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