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You are here:Home » Antibodies » Abs to Nitric Oxide » Anti -NOS III, phosphorylated (Ser1177) (Endothelial Nitric Oxide Synthase, eNOS, NOS 3)

Anti -NOS III, phosphorylated (Ser1177) (Endothelial Nitric Oxide Synthase, eNOS, NOS 3)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified E B
Endothelial NO synthase produces nitric axide (NO) which plays a crucial role in cardiovascular homeostasis. Activation of the enzyme and NO production can be induced by phosphorylation of eNOS at serine 1177 by activated Akt/PKB.
Catalog #N5350-26B
Applications
Suitable for use in ELISA and Western Blot. Other applications have not been tested.
Recommended Dilutions
Western Blot: 2ug/ml (HRP/ECL)
ELISA: 0.1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No6D618
HostMouse
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, 0.09% sodium azide, PEG, sucarose, 50% glycerol.
PurityPurified by size exclusion chromatography.
ImmunogenSynthetic peptide corresponding to a portion of human eNOS (endothelial nitric oxide synthase) phosphorylated at Ser1177.
SpecificityRecognizes human eNOS phosphorylated at Ser1177. Does not cross-react with non-phosphorylated eNOS or other unrelated serine-phosphorylated proteins. Species crossreactivity: mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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