Anti -NOS III, phosphorylated (Ser617) (Nitric Oxide Synthase 3, Nitric Oxide Synthase 3
Endothelial Cell, NOS Type III, NOS3, Endothelial Nitric Oxide Synthase 3, Endothelial
NOS, eNOS, ECNOS, EC-NOS, Constitutive NOS, cNOS, Nitric Oxide Synthase Endothelial)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
E B |
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| Catalog # | N5350-28E | | Applications | Suitable for use in ELISA and Western Blot. Other applications not tested. | | Recommended Dilution | Western Blot: 1:1000-1:2000 detects phosphorylated NOS III (Ser617) in RIPA lysates from fetal bovine heart endothelial (FBHE) cells. Lambda protein phosphatase treated and untreated FBHE cell lysates were resolved by electrophoresis, transferred to nitrocellulose and probed with N5350-28E (1:1000). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. | | Optimal dilutions to be determined by the researcher. | | Recommended Secondary Antibodies I1904-39 | IgG, X-Adsorbed (HRP) Pab Gt xRb | | I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb | | I1904-46Q: IgG, H&L (HRP) Pab Gt xRb | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Bovine | | Concentration | Not determined. | | Form | Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Synthetic peptide corresponding to amino acids surrounding phosphorylated Ser617 of bovine eNOS/NOS III (KLH). This site corresponds to human Ser615. | | Specificity | Recognizes bovine phosphorylated eNOS/NOS III (Ser617), Mr 132kD. A non-specific protein was also detected, Mr 70kD. Species sequence homology: human, mouse and canine. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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Download Specifications for Anti -NOS III, phosphorylated (Ser617) (Nitric Oxide Synthase 3, Nitric Oxide Synthase 3 Endothelial Cell, NOS Type III, NOS3, Endothelial Nitric Oxide Synthase 3, Endothelial NOS, eNOS, ECNOS, EC-NOS, Constitutive NOS, cNOS, Nitric Oxide Synthase Endothelial) |
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