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You are here:Home » Antibodies » Abs to Nitric Oxide » Anti -NOS III, phosphorylated (Ser617) (Nitric Oxide Synthase 3, Nitric Oxide Synthase 3 Endothelial Cell, NOS Type III, NOS3, Endothelial Nitric Oxide Synthase 3, Endothelial NOS, eNOS, ECNOS, EC-NOS, Constitutive NOS, cNOS, Nitric Oxide Synthase Endothelial)

Anti -NOS III, phosphorylated (Ser617) (Nitric Oxide Synthase 3, Nitric Oxide Synthase 3
Endothelial Cell, NOS Type III, NOS3, Endothelial Nitric Oxide Synthase 3, Endothelial
NOS, eNOS, ECNOS, EC-NOS, Constitutive NOS, cNOS, Nitric Oxide Synthase Endothelial)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Catalog #N5350-28E
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000-1:2000 detects phosphorylated NOS III (Ser617) in RIPA lysates from fetal bovine heart endothelial (FBHE) cells. Lambda protein phosphatase treated and untreated FBHE cell lysates were resolved by electrophoresis, transferred to nitrocellulose and probed with N5350-28E (1:1000). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies I1904-39 IgG, X-Adsorbed (HRP) Pab Gt xRb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb
I1904-46Q: IgG, H&L (HRP) Pab Gt xRb
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceBovine
ConcentrationNot determined.
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to amino acids surrounding phosphorylated Ser617 of bovine eNOS/NOS III (KLH). This site corresponds to human Ser615.
SpecificityRecognizes bovine phosphorylated eNOS/NOS III (Ser617), Mr 132kD. A non-specific protein was also detected, Mr 70kD. Species sequence homology: human, mouse and canine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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