Anti -NOS III, phosphorylated (Ser635) (Nitric Oxide Synthase 3, Nitric Oxide Synthase 3
Endothelial Cell, NOS Type III, NOS3, Endothelial Nitric Oxide Synthase 3, Endothelial
NOS, eNOS, ECNOS, EC-NOS, Constitutive NOS, cNOS, Nitric Oxide Synthase Endothelial)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
B |
|
| Catalog # | N5350-28F | | Applications | Suitable for use in Western Blot. Other applications not tested. | | Recommended Dilution | Western Blot: 1:2,000-1:5,000 detects phosphorylated eNOS/NOS III (Ser635) in RIPA lysates from fetal bovine heart endothelial (FBHE) cells. Lambda protein phosphatase treated and untreated FBHE cell lysates were resolved by electrophoresis, transferred to nitrocellulose and probed with antiphospho eNOS/NOS III (Ser635) (1:2,000). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Bovine | | Concentration | As reported | | Form | Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | KLH-conjugated, synthetic peptide corresponding to amino acids surrounding phosphorylated Ser635 of bovine eNOS/NOS III. This site corresponds to human Ser633. The immunizing sequence is identical in human and mouse, and has 11/12 identical amino acids in canine. | | Specificity | Recognizes phosphorylated eNOS/NOS III (Ser635), Mr 132kD. A non-specific protein was | | also detected, Mr 160kD. Species Crossreactivity | Bovine. Predicted to crossreact with human, mouse and canine based on sequence homology. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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Download Specifications for Anti -NOS III, phosphorylated (Ser635) (Nitric Oxide Synthase 3, Nitric Oxide Synthase 3 Endothelial Cell, NOS Type III, NOS3, Endothelial Nitric Oxide Synthase 3, Endothelial NOS, eNOS, ECNOS, EC-NOS, Constitutive NOS, cNOS, Nitric Oxide Synthase Endothelial) |
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