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You are here:Home » Antibodies » Abs to Nitric Oxide » Anti -NOS III (Nitric Oxide Synthase 3, Nitric Oxide Synthase 3 Endothelial Cell, NOS Type III, NOS3, Endothelial Nitric Oxide Synthase 3, Endothelial NOS, eNOS, ECNOS, EC-NOS, Constitutive NOS, cNOS, Nitric Oxide Synthase Endothelial)

Anti -NOS III (Nitric Oxide Synthase 3, Nitric Oxide Synthase 3 Endothelial Cell, NOS Type
III, NOS3, Endothelial Nitric Oxide Synthase 3, Endothelial NOS, eNOS, ECNOS, EC-NOS,
Constitutive NOS, cNOS, Nitric Oxide Synthase Endothelial)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Purified E IH
Catalog #N5350-28J
NOS oxidizes a guanidine nitrogen of arginine releasing nitric oxide in the form of a free radical and citrulline. Nitric oxide thus generated acts as a messenger in diverse functions including vasodilation neurotransmission, anti-tumor and anti-pathogenic activities. NOS is classified under three types neuronal NOS (nNOS) or brain NOS (bNOS); induciblr NOS (iNOS) or macrophage NOS (mNOS); and endothelial NOS (eNOS).
ApplicationsSuitable for use in Immunohistochemistry. Other applications not tested.
Recommended DilutionsImmunohistochemistry (Formalin/paraffin): 1:50 for 60 minutes. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by the researcher.
Positive ControlCapillary endothelium, regenerating endothelium
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in PBS, pH 7.6, 0.2% BSA, 15mM sodium azide.
PurityPurified
ImmunogenSynthetic peptide derived from C-terminal of human NOS III. Cellular Localization: Cytoplasmic.
SpecificityRecognizes human NOS III at ~135kD. Species Crossreactivity: mouse, rat, canine, porcine and bovine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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