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You are here:Home » Antibodies » Abs to Nitric Oxide » Anti -Nitric Oxide Synthase, Pan (bNOS, iNOS, eNOS, NOS I-III)

Anti -Nitric Oxide Synthase, Pan (bNOS, iNOS, eNOS, NOS I-III)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites E B IC
Nitric oxide (NO), a diffusible free radical gas, acts as a neurotransmitter in brain and peripheral nervous system. It accounts for the activity of endothelium-derived relaxing factors, which stimulate vasodilatation by releasing NO from the endothelium. NO is synthesized by L-arginine, oxygen, and NADPH by three known isoforms of heme- containing flavoproteins termed NO synthase (NOS, I-III, mol wt. ~130-160kD). One group of enzyme is constitutive, agonist-triggered, and dependent on Ca2+/Calmodulin and is inhibited by L-arginine analogues (L-NNA, L-NMMA). It is found in endothelium, adrenal glands, brain and platelets. The other principle group is inducible, Ca2+/Calmodulin-independent, and inhibited by NMMA and L-NNA. It has been found in macrophage, hepatocytes, tumor cells, vascular smooth muscle and endothelial cells. Analyses of cDNA clones have identified three distinct NOS genes in mammals: neuronal (nNOS/bNOS/NOS-I), endothelial (eNOS/NOS- III), and macrophage (mNOS/iNOS/NOS-II). Both nNOS and eNOS are constitutive and the mNOS/iNOS is inducible. Sequence homology among different cloned isoforms is ~50%. Human, rat, mouse, and bovine eNOS/NOS-3 are ~1202-1205aa proteins.
Catalog #N5350-29
ApplicationsSuitable for use in ELISA, Immunocytochemistry, and Western Blot. Other applications not tested.
Recommended DilutionImmunocytochemistry: 1-10ug/ml
Western Blot: 5-10ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgM
Clone No11C140
HostMouse
SourceBovine
ConcentrationAs reported
FormSupplied as a lyophilized powder from PBS, 0.05% sodium azide.
PurityAscites
ImmunogenHighly purified bovine brain NOS protein.
SpecificityRecognizes bovine NOS the three isoforms of nitric oxide synthase (NOS)
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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