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You are here:Home » Antibodies » Abs to Nitric Oxide » Anti -eNOS Phosphorylated (Ser1179) (NOS III, Nitric Oxide Synthase 3, Nitric Oxide Synthase 3 Endothelial Cell, NOS Type III, NOS3, Endothelial Nitric Oxide Synthase 3, Endothelial NOS, eNOS, ECNOS, EC-NOS, Constitutive NOS, cNOS, Nitric Oxide Synthase Endothe

Anti -eNOS Phosphorylated (Ser1179) (NOS III, Nitric Oxide Synthase 3, Nitric Oxide
Synthase 3 Endothelial Cell, NOS Type III, NOS3, Endothelial Nitric Oxide Synthase 3,
Endothelial NOS, eNOS, ECNOS, EC-NOS, Constitutive NOS, cNOS, Nitric Oxide Synthase
Endothe

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IF
The oxidation of L-arginine by a family of nitric oxide synthase (NOS) isoenzymes, nNOS, eNOS, and iNOS, is responsible for the production of nitric oxide (NO). In mammalian genomes, three separate genes encode the NOS isoforms, two expressed constitutively (nNOS, eNOS), and one inducible (iNOS). Endothelial NOS (eNOS, NOS III) is expressed in endothelial cells, some neurons and cardiac myocytes, and is responsible for cardiovascular homeostatic processes, including blood pressure regulation, vessel remodeling and angiogenesis (1).
eNOS undergoes a unique translocation cycle between the plasma membrane and the cytoplasm, suggesting that its intracellular localization is important in linking stimuli to eNOS enzymatic activity. Phosphorylation of the protein at several sites also plays a regulatory role. Phosphorylation of eNOS at Ser1179 in response to stimulation by VEGF has been observed to enhance eNOS activity, increasing the production of NO (1), while phosphorylation at Thr497 in combination with dephosphorylation at Ser1179 inhibits eNOS activity (2). Ser1179 (bovine)/Ser1177 (human) is the target residue for phosphorylation of multiple protein kinases, including Akt (3), AMP kinase (4), PKA and protein kinase G (5), and CaM kinase II (6).
Catalog #N5350-30
ApplicationsSuitable for use in Immunofluorescence, ELISA and Western Blot. Other applications not tested.
Recommended DilutionsELISA: 0.1-1ug/ml
Western Blot: 1-3ug/ml
Immuno uorescence: Reactive with monkey COS cells (1)
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceBovine
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to the C-terminal region of the bovine eNOS (endothelial nitric oxide synthase, NOS III) protein, phosphorylated at Ser1179.
SpecificityRecognizes bovine eNOS protein, phosphorylated at Ser1179 (~135kD). Reactivity has been con rmed with VEGF-stimulated, serum-starved BAEC (bovine aortic endothelial cell) lysates. A very low signal was observed in the lysates of serum-starved, unstimulated BAEC and no signal was observed in lysates of unstimulated BAEC in the presence of serum. Species Sequence Homology: human, rat, mouse, canine, porcine and guinea pig
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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