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You are here:Home » Antibodies » Abs to Enzymes, Oxidase » Anti -NOX5 (NADPH Oxidase 5, NADPH Oxidase, EF-hand Calcium Binding Domain 5, MGC149776, MGC149777, NOX5A, NOX5B)

Anti -NOX5 (NADPH Oxidase 5, NADPH Oxidase, EF-hand Calcium Binding Domain 5, MGC149776,
MGC149777, NOX5A, NOX5B)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
Calcium-dependent NADPH oxidase that generates superoxide. Also functions as a calcium-dependent proton channel and may regulate redox-dependent processes in lymphocytes and spermatozoa. May play a role in cell growth and apoptosis. Isoform v2 and isoform v5 are involved in endothelial generation of reactive oxygen species (ROS), proliferation and angiogenesis and contribute to endothelial response to thrombin.
Catalog #N5376-78
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:8000
Western Blot: 0.5-2ug/ml, human testis lysates observed on ~90kD bands
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to C-EWHPFTISSAPEQKD, from human NOX5, at the internal region of the protein (NP_078781.3, NP_001171708.1, NP_001171709.1).
SpecificityRecognizes human NOX5. Species sequence homology: Bovine and canine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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