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You are here:Home » Antibodies » Abs to Defensin » Anti -NP-1 (alpha Defensin-1)

Anti -NP-1 (alpha Defensin-1)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B N
Defensins are a large family of broad-spectrum antimicrobial peptides, identified originally in leukocytes of rabbits and humans. Defensins, cationic/polar peptides (30-35 aa; 3-4kD), are distinguished by a conserved tri-disulfides and a largely b-sheet structure. In leukocytes, these peptides are stored in cytoplasmic granules and are released into phagolysosomes where they contribute to the killing of engulfed microorganisms. Defensins are classified into two families designated alpha and beta based on distinctive, although similar, tri-disulfide linkages in the peptides. b-defensins are slightly larger and differ in the position and arrangement of 3 disulfides. In humans, six a-defensin (cryptidins), HD 1-6 (HD1-4 are also known as HNP1-4 for Human Neutrophil Peptides), and two b-defensins, HBD-1 and HBD-2, have been identified. Alpha-defensins are encoded by genes designated DEFA1-6, whereas human b-defensins are encoded by the DEFB1 and DEFb2 genes. HD1-4 are expressed in neutrophils, whereas HD5 and HD6 are expressed in epithelial cells of the intestinal and reproductive tract. HD1-3/HNP1-3 make up about 30% of the neutrophil's total granule protein. The HNPs are 29 to 30 amino acids long and are identical in sequence except at the N-terminal amino acid.
Catalog #N5377-01
ApplicationsSuitable for use in ELISA, Neutralization and Western Blot. Other applications have not been tested.
Recommended Dilution  
ELISA (indirect or Sandwich): 0.5-2.0ug/ml. Use 100ul/well antibody solution. Detection limit: 0.2-0.4ng/well.
Neutralization: 0.40-0.60ug/ml yeilds one-half maximum inhibition [ND50] of the biological activity.
Western Blot:  0.1-0.2ug/ml. Detection limit for rhNP-1 is 1.53.0ng/lane, under either reducing or non-reducing conditions.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile ddH2O containing 40-50% glycerol. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Country of Origin USA
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
ConcentrationNot determined
FormSupplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with sterile ddH2O, 40-50% glycerol to a concentration of 0.1-1mg/ml.
PurityPuri ed by immunoaf nity chromatography.
ImmunogenHighly pure (>98%) recombinant hNP-1.
SpecificityRecognizes human NP-1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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