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You are here:Home » Antibodies » Abs to Defensin » Anti -NP-1 (alpha Defensin-1) (Biotin)

Anti -NP-1 (alpha Defensin-1) (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
Alpha-defensin-1 (NP-1) is an antimicrobial peptide which belongs to the distinct family of alpha-defensins. Recombinant Human NP-1 is a 3.4kD protein containing 30 amino acid residues.
Catalog #N5377-02
Defensins are a large family of broad-spectrum antimicrobial peptides, identified originally in leukocytes of rabbits and humans. Defensins, cationic/polar peptides (30-35 aa; 3-4kD), are distinguished by a conserved tri-disulfides and a largely b-sheet structure. Defensins, expressed at the cell surface, have been hypothesized to function as a biochemical barrier against microbial infection by inhibiting colonization of the epithelium by wide range of pathogenic microorganisms. In leukocytes, these peptides are stored in cytoplasmic granules and are released into phagolysosomes where they contribute to the killing of engulfed microorganisms. The genes encoding human alpha and beta-defensins are clustered in a contiguous segment of chromosome 8p23. Defensins are classified into two families designated alpha and beta based on distinctive, although similar, tri-disulfide linkages in the peptides. b-defensins are slightly larger and differ in the position and arrangement of 3 disulfides. In humans, six a-defensin (cryptidins), HD 1-6 (HD1-4 are also known as HNP1-4 for Human Neutrophil Peptides), and two b-defensins, HBD-1 and HBD-2, have been identified.
Alpha-defensins are encoded by genes designated DEFA1-6, whereas human b-defensins are encoded by the DEFB1 and DEFb2 genes. HD1-4 are expressed in neutrophils, whereas HD5 and HD6 are expressed in epithelial cells of the intestinal and reproductive tract. HD1-3/HNP1-3 make up about 30% of the neutrophil's total granule protein. The HNPs are 29 to 30 amino acids long and are identical in sequence except at the N-terminal amino acid. DEFA1 and DEFA3 differ from each other only by their terminal ala and asp, respectively. DEFA2 is identical to both except that it has 29 instead of 30 amino acids.
Applications  
ELISA:  To detect hNP-1 by direct ELISA (using 100ul/well antibody solution) this antibody can be used at a concentration of 0.150.30ug/ml. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2ng/well of recombinant hNP-1.
Western Blot To detect hNP-1 by Western Blot analysis this antibody can be used at a concentration of 0.10.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNP-1 is 1.5 3.0ng/lane, under either reducing or non-reducing conditions.
Stability The lyophilized antibody is stable for at least 6 months when kept at 20°C.
Reconstitution The biotinylated antibody should be reconstituted to a concentration of 50ug/ml with sterile PBS solution containing 0.1% BSA. This solution can be stored at 4°C for at least one month without detectable loss of activity. Frozen aliquots of this solution are stable for at least 6 months when kept at-20°C. Avoid more than one freeze-thaw cycle.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
FormLyophilized from sterile- ltered PBS solution.
PurityPuri ed by af nity chromatography and then biotinylated.
ImmunogenHighly pure (>98%) recombinant hNP-1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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