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You are here:Home » Antibodies » Abs to Defensin » Anti -NP-1 (alpha Defensin-1)

Anti -NP-1 (alpha Defensin-1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum E B
Defensins are a large family of broad-spectrum antimicrobial peptides. There were oringinally identified in leukocytes of rabbits and humans. Defensins are cationic/polar peptides (30-35aa; 3-4kD). They are distinguished by a conserved tri-disulfides and a largely b-sheet structure. Defensins expressed at the cell surface have been hypothesized to function as a biochemical barrier against microbial infection by inhibiting colonization of the epithelium by wide range of pathogenic microorganisms. In leukocytes, these peptides are stored in cytoplasmic granules. These granules are released into phagolysosomes where they contribute to the killing of engulfed microorganisms. The genes encoding human alpha- and beta-defensins are clustered in a contiguous segment of chromosome 8p23. Defensins are classified into two families designated alpha and beta based on distinctive, although similar, tri-disulfide linkages in the peptides. b-defensins are slightly larger and differ in the position and arrangement of 3 disulfides. In humans, six a-defensin (cryptidins), HD 1-6 and two b-defensins, HBD-1 and HBD-2, have been identified. HD1-4 are also known as HNP1-4 for Human Neutrophil Peptides. Alpha-defensins are encoded by genes designated DEFA1-6. Human b-defensins are encoded by the DEFB1 and DEFb2 genes. HD1-4 are expressed in neutrophils,. HD5 and HD6 are expressed in epithelial cells of the intestinal and reproductive tract. HD1-3/HNP1-3 make up about 30% of the neutrophil's total granule protein. The HNPs are 29-30aa in length. They are identical in sequence except at the N-terminal amino acid. DEFA1 and DEFA3 differ from each other only by their terminal ala and asp, respectively. DEFA2 is identical to both except that it has 29 instead of 30 amino acids.
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
Western Blot: 1:1000-1:5000
ELISA: 1:10,000-50,000. Coated with 1ug/ml Control Peptide/ well (Catlalog #N5377-02F).
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalog #N5377-02G
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot Determined
FormSupplied as a liquid in PBS, 0.05% sodium azide.
PuritySerum
ImmunogenHuman alpha defensin-1 (HDEFA1) was synthesized from 94aa precursor protein (mature peptide 65-94aa). Synthetic peptide corresponding to 16aa near the C-terminus of human mature alpha defensin-1 (KLH).
SpecificityRecognizes human alpha defensin-1. No sequence homology with human alpha-defensins 4-6.
Species sequence homologyMonkey alpha-defensins 1-3.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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