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You are here:Home » Antibodies » Abs to GTPase, Rab, Ras, Rho Proteins » Anti -NRAS (GTPase NRas, Transforming Protein N-Ras, HRAS1)

Anti -NRAS (GTPase NRas, Transforming Protein N-Ras, HRAS1)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified B IH
This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated to a GTP-bound form by a GTPase activating protein and inactivated to a GDP-bound form by a guanine nucleotide-exchange factor. Defects in this gene are a cause of juvenile myelomonocytic leukemia (JMML).
Catalog #N5385-01D
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 0.01-0.03ug/ml. A band at ~21kD is seen with human lysates in A431 cell line. In transfected HEK293 transiently expressing NRAS bands of ~21kD and 25kD are seen. These bands are not seen in non-transfected HEK293.
Immunohistochemistry (Paraffin): 2-3ug/ml. Shows staining of cytoplasm and membranes of enterocytes in paraffin embedded human small intestine.
Optimal dilutions to be determined by the researcher.
Positive ControlHuman lysates of cell line A431
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to C-RMKKLNSSDDGTQ, from the internal region of the protein sequence according to NP_002515.1.
SpecificityRecognizes human NRAS. Species Sequence Homology: canine, mouse and rat
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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