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You are here:Home » Antibodies » Abs to Enzymes, ATPase » Anti -Nsf (Vesicle-fusing ATPase, N-ethylmaleimide-sensitive Fusion Protein, NEM-sensitive Fusion Protein, Vesicular-fusion Protein NSF, Protein SKD2, Skd2)

Anti -Nsf (Vesicle-fusing ATPase, N-ethylmaleimide-sensitive Fusion Protein, NEM-sensitive
Fusion Protein, Vesicular-fusion Protein NSF, Protein SKD2, Skd2)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP IH
Syntaxins were originally thought to be docking proteins, but have more recently been categorized as anchoring proteins that anchor themselves to the cytoplasmic surfaces of cellular membranes. Syntaxins have been shown to bind to various proteins involved in exocytosis, including VAMPs (vesicle-associated membrane proteins), NSF (N-ethylmaleimidesensitive factor), SNAP 25 (synaptosomal-associated protein of 25kD), SNAPs (soluble NSF attachment proteins) and synaptotagmin. SNAPs mediate the membrane binding of NSF, which is essential for membrane fusion reactions. An additional protein designated synaptophysin may regulate exocytosis by competing with SNAP 25 and syntaxins for VAMP binding.
Catalog #N5396-01C
ApplicationsSuitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1-2ug/ml
Immunoprecipitation: 5-15ug/ml
Immunohistochemistry: 10-20ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeLgG
HostRabbit
SourceMouse
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, 1% BSA, 0.02% sodium azide, 50% glycerol.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide corresponding to aa731 from mouse NSF.
SpecificityRecognizes mouse NSF at ~85kD. Species Crossreactivity: human, mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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