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You are here:Home » Molecular Biology » MB-Neurotrophins » NT-1, Recombinant, Human (Neurotrophin 1/B Cell Stimulating Factor 3, BCSF-3)

NT-1, Recombinant, Human (Neurotrophin 1/B Cell Stimulating Factor 3, BCSF-3)

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Specifications

Human NT-1/BCSF-3 (Neurotrophin-1/B-Cell Stimulating Factor-3) is identi ed as part of the IL-6 family of cytokines. It is most homologous to cardiotrophin-1 and ciliary neurotrophic factor. NT-1/BCSF-3 is a neurotrophic factor with B-cell stimulating capabilities. Expressed in lymph nodes and spleen, NT-1/BCSF-3 binds and activates glycoprotein 130 (gp130) and leukemia inhibitory factor receptor member beta (LIFR-b) and induces tyrosine phosphorylation of these receptors. In vitro, it supports survival of chick embryo motor and sympathetic neurons. In mice, NT-1/BCSF-3 includes serum amyloid A, causes body weight loss and B cell hyperplasia associated with increase in serum IgG and IgM. Recombinant human NT-1/BCSF-3 is a 22.4kD protein containing 199 amino acid residues.
Catalog #N5399
AA SequenceMLNRTGDPGP GPSIQKTYDL TRYLEHQLRS LAGTYLNYLG PPFNEPDFNP PRLGAETLPR ATVDLEVWRS LNDKLRLTQN YEAYSHLLCY LRGLNRQAAT AELRRSLAHF CTSLQGLLGS IAGVMAALGY PLPQPLPGTE PTWTPGPAHS DFLQKMDDFW LLKELQTWLW RSAKDFNRLK KKMQPPAAAV TLHLGAHGF
Endotoxin 0.1ng/ug (1EU/ug)
Biological ActivityNT-1/BCSF-3 weakly supports chick E8 DRG neurite outgrowth at a concentration of 1.0ng/ml.
ReconstitutionReconsitute with water to a concentration of 0.1-1.0mg/ml.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile water and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Country of OriginUSA
SourceE. coli
Purity98% by SDS-PAGE and HPLC analyses
ConcentrationAs reported
FormSupplied as a lyophilized powder
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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