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You are here:Home » Antibodies » Abs to Neurotrophins » Anti -NT-1 (Neurotrophin 1, B Cell Stimulating Factor 3, BCSF-3)

Anti -NT-1 (Neurotrophin 1, B Cell Stimulating Factor 3, BCSF-3)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Catalog #N5399-05
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionIndirtect and Sandwich ELISA: > 0.5ug/ml using 100ul/well antibody solution detects 0.2-0.4ng/well of recombinant hNNT-1/BCSF-3.
Western Blot: 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit is 1.5-3.0ng/lane, under either reducing or non-reducing conditions
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol, ddH2O, and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with 40-50% glycerol, sterile dH2O to 0.1-1mg/ml.
PurityPurified by immunoaffinity chromatography.
ImmunogenHighly pure (> 98%) recombinant human NT-1 (novel)/BCSF-3.
SpecificityRecognizes human NT-1 (novel)/BCSF-3.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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