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You are here:Home » Molecular Biology » MB-Neurotrophins » NT-3, Recombinant, Human (Neurotrophin 3, Neurotrophin-3, NT3, NTF3, HDNF, MGC129711, Nerve Growth Factor 2, Nerve Growth Factor-2, NGF2, NGF-2, Neurotrophic Factor)

NT-3, Recombinant, Human (Neurotrophin 3, Neurotrophin-3, NT3, NTF3, HDNF, MGC129711,
Nerve Growth Factor 2, Nerve Growth Factor-2, NGF2, NGF-2, Neurotrophic Factor)

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Specifications

NT-3 is a neurotrophic factor structurally related to NGF, BDNF, and NT-4. These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. NT-3 is expressed by neurons of the central nervous systems and can signal through the trk receptors. NT-3 promotes the growth and survival of nerve and glial cells. The amino acid sequences of human, murine and rat NT-3 are identical.
Catalog #N5400-01
DescriptionRecombinant Human NT-3 is a single, non-glycosylated polypeptide chain containing 119 amino acids and having a molecular mass of 13606.29 Dalton.
SequenceThe sequence of the first five N-terminal amino acids was determined and was found to be Tyr-Ala-Glu-His-Lys.
Biological ActivityThe ED50 as determined by the dose-dependent induction of choline acetyl transferase in rat basal forebrain primary septal culture was found between 20-50ng/ml.
Protein ContentProtein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 2.165 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of NT-3 as a Reference Standard.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
SourceE. coli
Purity95% by RP-HPLC or SDS-PAGE. Chromatographically purified.
Concentration~0.1mg/ml
FormSupplied as a lyophilized powder. No additives. Reconstitute with ddH2O to 0.1mg/ml, which can then be further diluted to other aqueous solutions.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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