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You are here:Home » Antibodies » Abs to Neurotrophins » Anti -NT-3 (Neurotrophin 3, HDNF, NGF-2, Nerve Growth Factor-2) (Biotin)

Anti -NT-3 (Neurotrophin 3, HDNF, NGF-2, Nerve Growth Factor-2) (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
Catalog #N6000-06Z
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: To detect hNT-3 by direct ELISA (using 100ul/well antibody solution) this antibody can be used at a concentration of 0.15-0.30ug/ml. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2ng/well of recombinant hNT-3.
Western Blot: To detect hNT-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNT-3 is 1.5-3.0ng/lane, under either reducing or non-reducing conditions.
Optimal dilutions to be determined by the researcher.
ReconstitutionReconstitute to a concentration of 50ug/ml with sterile PBS solution containing 0.1% BSA, 40-50% glycerol.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Country of OriginUSA
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.05mg/ml
FormSupplied as a lyophilized powder from a sterile-filtered PBS solution. Conjugated to Biotin.
PurityPurified by immunoaffinity chromatography.
ImmunogenHighly pure (> 98%) recombinant human Neurotrophin-3
SpecificityRecognizes human Neurotrophin-3.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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