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Anti -NT-3 (Neurotrophin 3, HDNF, NGF-2, Nerve growth factor-2)
Anti -NT-3 (Neurotrophin 3, HDNF, NGF-2, Nerve growth factor-2)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Goat |
Affinity Purified |
E B N |
|
| NT-3 is a neurotrophic factor structurally related to NGF, BDNF, and NT-4. These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. NT-3 is expressed by neurons of the central nervous systems and can signal through the trk receptors. NT-3 promotes the growth and survival of nerve and glial cells. The amino acid sequences of human, murine and rat NT-3 are identical. Recombinant human NT-3 is a noncovalently linked homodimer, of two 13.6kD polypeptide monomers (238 total amino acid residues). | | | Catalog # | N6000-07 | | Applications | Suitable for use in Neutralization, ELISA, and Western Blot. Other applications not tested. | | Recommended Dilution | Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity (50ng/ml), a concentration of 2-4ug/ml of this antibody is required. | | ELISA | Detection by direct ELISA (using 100ul/well antibody solution), requires a concentration of at least 0.5ug/ml of antibody. This antigen-affinity purified antibody, in conjunction with compatible secondary reagents, allows detection of 0.2-0.4ng/well. | | Western Blot | Use at a concentration of 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit is 1.5-3.0ng/lane, under either reducing or non-reducing conditions. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Goat | | Source | Human | | Concentration | 1mg/ml | | Form | Supplied as a lyophilized powder from 0.5X PBS, pH 7.4. | | Purity | Purified by immobilized antigen affinity chromatography. | | Immunogen | Highly purified (>98%) recombinant antigen. | | Specificity | Recognizes NT-3 | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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