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You are here:Home » Antibodies » Abs to Neurotrophins » Anti -NT-3 (Neurotrophin 3, HDNF, NGF-2, Nerve growth factor-2)

Anti -NT-3 (Neurotrophin 3, HDNF, NGF-2, Nerve growth factor-2)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B N
NT-3 is a neurotrophic factor structurally related to NGF, BDNF, and NT-4. These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. NT-3 is expressed by neurons of the central nervous systems and can signal through the trk receptors. NT-3 promotes the growth and survival of nerve and glial cells. The amino acid sequences of human, murine and rat NT-3 are identical. Recombinant human NT-3 is a noncovalently linked homodimer, of two 13.6kD polypeptide monomers (238 total amino acid residues).
Catalog #N6000-07
ApplicationsSuitable for use in Neutralization, ELISA, and Western Blot. Other applications not tested.
Recommended DilutionNeutralization: To yield one-half maximal inhibition [ND50] of the biological activity (50ng/ml), a concentration of 2-4ug/ml of this antibody is required.
ELISA Detection by direct ELISA (using 100ul/well antibody solution), requires a concentration of at least 0.5ug/ml of antibody. This antigen-affinity purified antibody, in conjunction with compatible secondary reagents, allows detection of 0.2-0.4ng/well.
Western Blot Use at a concentration of 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit is 1.5-3.0ng/lane, under either reducing or non-reducing conditions.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration1mg/ml
FormSupplied as a lyophilized powder from 0.5X PBS, pH 7.4.
PurityPurified by immobilized antigen affinity chromatography.
ImmunogenHighly purified (>98%) recombinant antigen.
SpecificityRecognizes NT-3
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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