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You are here:Home » Antibodies » Abs to Neurotrophins » Anti -NT-3 (Neurotrophin 3, HDNF, NGF-2, Nerve growth factor-2)

Anti -NT-3 (Neurotrophin 3, HDNF, NGF-2, Nerve growth factor-2)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
NT-3 is a neurotrophic factor structurally related to NGF, BDNF, and NT-4. These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. NT-3 is expressed by neurons of the central nervous systems and can signal through the trk receptors. NT-3 promotes the growth and survival of nerve and glial cells. The amino acid sequences of human, murine and rat NT-3 are identical. Recombinant human NT-3 is a noncovalently linked homodimer, of two 13.6kD polypeptide monomers (238 total amino acid residues).
Catalog #N6000-07E
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 0.5-2ug/ml
Immunohistochemistry: 0.5-2ug/ml
Optimal dilutions to be determined by the researcher.
Reconstitution Desired volume with sterile distilled water.
Suggested concentration 0.51mg/mL.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
Concentration~0.5-1mg/ml
FormSupplied as a lyophilized powder in PBS. No preservatives added.
PurityPurified by immunoaffinity chromatography.
ImmunogenAn 11 amino acid peptide corresponding to the amino-terminal of mouse Neurotrophin 3 (NT3) coupled to BSA.
SpecificityRecognizes Neurotrophin 3 (NT3). Does not crossreact with BDNF or NGF. Species Reactivity: Human, rat, chicken.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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