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Anti -Nuclear Pore Complex, nup153 (NPC)
Anti -Nuclear Pore Complex, nup153 (NPC)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Supernatant |
B IP IH IF |
|
| Nuclear pore complexes are extremely elaborate structures that mediate the regulated movement of macromolecules between the nucleus and cytoplasm. These complexes are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. | | | Catalog # | N6888-85 | | Applications | Suitable for use in Western Blot, Immunoprecipitation, Immunofluorescence microscopy and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Immunohistochemistry: 1:5-1:10; Incubation time 1 hr at RT (frozen sections; also after fixation with paraformaldehyde). | | Western Blot: 1:30-1:50 (ECL method). | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 5F280 | | Host | Mouse | | Source | Rat | | Concentration | Not determined | | Form | Supplied as a liquid, 0.09% sodium azide. | | Purity | Supernatant | | Immunogen | Nuclear matrix protein fraction prepared from rat liver nuclei. | | Specificity | Recognizes the nuclear pore complex (NPC) protein nup153 (190kD), present on the inner aspect of NPC with attached filament bundles. It is also reactive with the soluble form of nup153 found e.g. during meiotic metaphase. | | Species Crossreactivity | Human, rodents (mouse, rat), marsupials (kangaroo rat), amphibia (xenopus, pleurodeles). | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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