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You are here:Home » Antibodies » Abs to Nuclear Proteins » Anti -Nuclear Pore Complex, Yeast (NPC)

Anti -Nuclear Pore Complex, Yeast (NPC)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E IF
The nuclear pore complex (NPC) has a molecular mass of 125,000 Da in vertebrates and contains about 50 or more different proteins. The NPC spans the dual membrane of the nuclear envelope (NE) and acts as a gateway for macromolecular traffic between the cytoplasm and the nucleus. The basic framework of the NPC consists of a central core with a ring-spoke structure exhibiting eightfold radial symmetry. From this central ring 50 to 100 nm fibrils extend into the nucleoplasm and the cytoplasm. The NPC is in turn anchored in the NE by the nuclear lamina, a meshwork of lamins and lamin-associated proteins that forms a 15 nm thick fibrous structure between the inner nuclear membrane and peripheral chromatin. A number of proteins called nucleoporins (Nups) have been localized to discrete regions of the NPC and are often used as markers for this compartment, e.g. Nup153. Approximately half of the nucleoporins contain a phenyalanine-glycine repeat motif (FG repeat), which may be diagnostic for proteins playing a role in nuclear transport. This is a reliable general purpose monoclonal antibody that recognizes a related family of NPC proteins. This antibody is ideal for studying the morphology and composition of the nucleus and nuclear envelope. It is also useful in studying changes in the nuclear structure during mitosis and meiosis. Ig Subclass Mouse monoclonal (IgG1)
Catalog #N6888-86Y
ApplicationsSuitable for use in ELISA and Immunofluorescence. Other applications not tested.
Recommended DilutionImmunofluorescence on yeast cells: 1:50-1:250
Immunofluorescence on mammalian cells: 1:25-1:50
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No8A275
HostMouse
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenYeast nuclear preparations
SpecificityRecognizes panspecific nuclear pore complex protein, useful nuclear pore complex and nuclear envelope marker in all eukaryotes tested to date.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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