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You are here:Home » Antibodies » Abs to Nuclear Proteins » Anti -Nuclear Spliceosomes

Anti -Nuclear Spliceosomes

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Supernatant B IP IF
A spliceosome is a complex of specialized RNA and protein subunits that removes introns from a transcribed pre-mRNA (hnRNA) segment. This process is generally referred to as splicing.
Catalog #N6895
ApplicationsSuitable for use in Indirect Immunofluorescence, Western Blot and Immunoprecipitation. Other applications have not been tested.
Recommended DilutionsIndirect immunofluorescence: 1:20-1:30 Use 30-100ul per well/slide.
Western Blot: Detects an ~105kD protein from human cells.
Immunoprecipitation: Immunoprecipates spliceosome and level sn RNPs 1,2,5 (using 0.5M salt).
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgM
Clone No2Q2236
HostMouse
SourceHuman
ConcentrationNot Determined
FormSupplied as a liquid in PBS, 0.1% sodium azide
PuritySupernatant
ImmunogenNuclear Spliceosomes
SpecificityRecognizes human nuclear spliceosomes. Using indirect immunofluorescence, produces a speckled nuclear staining patter. Immunoelectron microscopy has shown that the antibody recognizes a discrete area of the nucleus identified as interchromatin granules.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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