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Anti -Nuclei and Chromosomes, Histone specific
Anti -Nuclei and Chromosomes, Histone specific
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Purified |
E IH IC IF |
|
| Catalog # | N6927 | | Applications | Suitable for use in ELISA, Immunocytochemistry, Indirect Immunofluorescence and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Immunocytochemistry (Indirect immunofluorescence): 1:20-1:30, 30-100ul per slide/well using paraformaldehyde/acetone fixed cell preparation. | | Immunohistochemistry | Formalin fixed, paraffin embedded tissue sections or paraformaldehyde/acetone fixed frozen tissue sections. | | Optimal dilution determined by the researcher. | | Positive Control | Human tonsil | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2a | | Clone No | 2Q2232 | | Host | Mouse | | Source | Human | | Concentration | Not determined | | Form | Supplied as a liquid in PBS, 0.1% sodium azide. | | Purity | Purified by ammonium sulfate precipitation. | | Specificity | Histone specific; stains nuclei and chromosomes of cells of all species including animals and plants. Reacts preferentially with Histone H1 by ELISA and immunoblot. Produces a speckled nuclear staining pattern in all tissues studies. Stains nuclei of all human cell types and also stains chromosomes diffusely in metaphase cells. Nuclear staining in interphase cells is intense and diffuse. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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