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You are here:Home » Antibodies » Abs to Nuclear Proteins » Anti -Nuclei and Chromosomes, Histone specific

Anti -Nuclei and Chromosomes, Histone specific

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified E IH IC IF
Catalog #N6927
ApplicationsSuitable for use in ELISA, Immunocytochemistry, Indirect Immunofluorescence and Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunocytochemistry (Indirect immunofluorescence): 1:20-1:30, 30-100ul per slide/well using paraformaldehyde/acetone fixed cell preparation.
Immunohistochemistry Formalin fixed, paraffin embedded tissue sections or paraformaldehyde/acetone fixed frozen tissue sections.
Optimal dilution determined by the researcher.
Positive ControlHuman tonsil
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No2Q2232
HostMouse
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in PBS, 0.1% sodium azide.
PurityPurified by ammonium sulfate precipitation.
SpecificityHistone specific; stains nuclei and chromosomes of cells of all species including animals and plants. Reacts preferentially with Histone H1 by ELISA and immunoblot. Produces a speckled nuclear staining pattern in all tissues studies. Stains nuclei of all human cell types and also stains chromosomes diffusely in metaphase cells. Nuclear staining in interphase cells is intense and diffuse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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