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You are here:Home » Antibodies » Abs to Nuclear Proteins » Anti -Nuclei and Chromosomes, Histone specific

Anti -Nuclei and Chromosomes, Histone specific

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified E B IH IF
Catalog #N6927-01
ApplicationsSuitable for use in ELISA, Immunofluorescence, Immunohistochemistry (frozen and paraffin), and Western Blot. Other applications not tested.
Recommended DilutionImmunofluorescence: 1:20-1:30
Optimal dilutions to be determined by the researcher.
Positive ControlHuman Tonsil
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No10F247
HostMouse
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
PurityPurified by ammonium sulfate precipitation.
ImmunogenNative.
SpecificityHistone specific, reacting preferentially with histone H1, a nuclear protein necessary for the condensation of nucleosome chains into higher order structures. This antibody stains nuclei of all human cell types and also stains chromosomes diffusely in metaphase cells. Intense diffuse staining is obtained in interphase cells.Species crossreactivity: rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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