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You are here:Home » Antibodies » Abs to Nuclear Proteins » Anti -Nucleolar Protein, 30kD (NO30, Nop30)

Anti -Nucleolar Protein, 30kD (NO30, Nop30)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Apoptosis, also known as programmed cell death, plays major roles in development and normal tissue turnover in addition to tumor formation (1 for review). Apoptosis is regulated by death domain (DD) and/or caspase recruitment domain (CARD) containing molecules and the caspase family of proteases. CARD domain containing cell death regulators include RAIDD, Apaf-1, caspase-9, and caspase-2. A novel CARD domain containing protein was recently identified and designated ARC for apoptosis repressor with CARD (3). An alternate splicing isoform of ARC was identified as Nop30 (3). While ARC interacts with caspase-2 and 8 and suppresses apoptosis induced by cell death adapters FADD and TRADD and by cell death receptors Fas, TNFR-1 and DR3 (2), Nop30 multimerizes and binds to the splicing factor SRp30c and may act to influence alternative splice site selection in vivo (3).
Catalog #N6939-69B
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 0.5-1ug/ml. See N6939-69C for control peptide.
Optimal dilutions to be determined by the researcher.
Positive ControlMouse skeletal tissue lysate
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, 0.02% sodium azide.
PurityPurified by immunoaffinity chromatography.
Immunogen14 amino acid peptide from near the carboxy terminus of human Nop30 (GenBank accession no. AAH12798)
SpecificityRecognizes human Nop30. Will not detect ARC protein. Species Crossreactivity: Mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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