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You are here:Home » Antibodies » Abs to Nuclear Proteins » Anti -Nucleolar Protein, 38kD (NO38)

Anti -Nucleolar Protein, 38kD (NO38)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites B IP IH IF
Catalog #N6939-70
ApplicationsSuitable for use in Immunoblot (Western), Immunoprecipitatioon, Immunofluorescence microscopy and Immunohistochemistry (frozen sections, also after fixation with paraformaldehyde).
Recommended DilutionWestern Blot: 1:1000-1:2000 (ECL method)
Immunohistochemistry: 1:300-1:500; Incubation time 1 hr at RT.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No5F277
HostMouse
SourceXenopus
ConcentrationNot determined
FormSupplied as a liquid with 0.09% sodium azide.
PurityAscites
ImmunogenNucleolar fraction prepared from Xenopus laevis oocytes.
SpecificityRecognizes a 38kD nucleolar protein (NO38) in Xenopus (this protein has also been termed B23, nucleophosmin, numatrin in other species). Stains specifically the granular component of the nucleolus and small nucleolar dots in interphase cells. During mitosis the protein is localized accumulated around the condensed chromosomes. Species Crossreactivity: Xenopus laevis, Xenopus borealis, Pleurodelis waltl, Triturus cristatus; XLKE-A6 cells; chicken; negative in higher animals.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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