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Specifications

Clone Host Grade Applications Monoclonal Mouse Ascites B IP IH IF

Catalog #	N6939-70
Applications	Suitable for use in Immunoblot (Western), Immunoprecipitatioon, Immunofluorescence microscopy and Immunohistochemistry (frozen sections, also after fixation with paraformaldehyde).
Recommended Dilution	Western Blot: 1:1000-1:2000 (ECL method)
	Immunohistochemistry: 1:300-1:500; Incubation time 1 hr at RT.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	5F277
Host	Mouse
Source	Xenopus
Concentration	Not determined
Form	Supplied as a liquid with 0.09% sodium azide.
Purity	Ascites
Immunogen	Nucleolar fraction prepared from Xenopus laevis oocytes.
Specificity	Recognizes a 38kD nucleolar protein (NO38) in Xenopus (this protein has also been termed B23, nucleophosmin, numatrin in other species). Stains specifically the granular component of the nucleolus and small nucleolar dots in interphase cells. During mitosis the protein is localized accumulated around the condensed chromosomes. Species Crossreactivity: Xenopus laevis, Xenopus borealis, Pleurodelis waltl, Triturus cristatus; XLKE-A6 cells; chicken; negative in higher animals.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.