Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications Monoclonal Mouse Ascites B IP IH IF

Catalog #	N6939-71
Applications	Suitable for use in Western Blot, Immunoprecipitation, Immunofluorescence and Immunohistochemistry. Other applications not tested.
Recommended Dilution	Western Blot: 1:1000-1:2000 (ECL method) Nitrocellulose sheet are better then nylon sheets.
	Immunohistochemistry: 1:300-1:500; (frozen sections, also after fixation with paraformaldehyde).
	Immunoprecipitation: from Xenopus oocyte extracts nucleoplasmin-a 30kD protein-is co-precipitated, which shows high sequence homology to NO38.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a
Clone No	5F279
Host	Mouse
Source	Xenopus
Concentration	Not determined
Form	Supplied as a liquid, 0.09% sodium azide.
Purity	Ascites
Immunogen	Nucleolar fraction prepared from Xenopus laevis oocytes.
Specificity	Recognizes a 38kD nucleolar protein (NO38) in Xenopus (this protein has also been termed B23, nucleophosmin, numatrin in other species). Stains specifically the granular component of the nucleolus and small nucleolar dots in interphase cells. During mitosis the protein is localized accumulated around the condensed chromosomes. Species Crossreactivity: Xenopus laevis, Xenopus borealis, Pleurodeles walt , Triturus cristatus; XLKE-A6 cells; chicken.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.