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You are here:Home » Antibodies » Abs to Nuclear Proteins » Anti -Nucleolar Protein, 38kD (NO38)

Anti -Nucleolar Protein, 38kD (NO38)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites B IP IH IF
Catalog #N6939-71
ApplicationsSuitable for use in Western Blot, Immunoprecipitation, Immunofluorescence and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000-1:2000 (ECL method) Nitrocellulose sheet are better then nylon sheets.
Immunohistochemistry: 1:300-1:500; (frozen sections, also after fixation with paraformaldehyde).
Immunoprecipitation: from Xenopus oocyte extracts nucleoplasmin-a 30kD protein-is co-precipitated, which shows high sequence homology to NO38.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No5F279
HostMouse
SourceXenopus
ConcentrationNot determined
FormSupplied as a liquid, 0.09% sodium azide.
PurityAscites
ImmunogenNucleolar fraction prepared from Xenopus laevis oocytes.
SpecificityRecognizes a 38kD nucleolar protein (NO38) in Xenopus (this protein has also been termed B23, nucleophosmin, numatrin in other species). Stains specifically the granular component of the nucleolus and small nucleolar dots in interphase cells. During mitosis the protein is localized accumulated around the condensed chromosomes. Species Crossreactivity: Xenopus laevis, Xenopus borealis, Pleurodeles walt , Triturus cristatus; XLKE-A6 cells; chicken.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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