You are here:Home
»
Antibodies
»
Abs to Nuclear Proteins
»
Anti -Nucleolar Protein, 180kD (xNopp180)
Anti -Nucleolar Protein, 180kD (xNopp180)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Supernatant |
B IP IH IF |
|
| Nucleolar Protein xNopp180 is a constitutively expressed nucleolar component and a specific marker for the dense fibrillar component of the interphase nucleolus. | | | Catalog # | N6939-76 | | Applications | Suitable for use in Immunoprecipitation, Immunoblot (Western), Immunofluorescence microscopy and Immunohistochemistry (frozen sections, also after fixation with paraformaldehyde) | | | | Recommended Dilution: | | Western Blot: 1:50 (ECL method) | | Immunohistochemistry: 1:5-1:10; Incubation time 1 hr at RT. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 5F276 | | Host | Mouse | | Source | Xenopus | | Concentration | Not determined | | Form | Supplied as a liquid in 0.09% sodium azide. | | Purity | Hybridoma culture supernatant | | Immunogen | Nucleolar fraction prepared from Xenopus laevis oocytes. | | Specificity | Stains specifically the nucleolus and Cajal bodies in interphase cells. Recognizes 180kD polypeptide in Xenopus (xNopp180), homolog to the rat polypeptide (Nopp140). When applied at high concentration to human cells the antibody reacts with a nucleolar component which, however, does not correspond to xNopp180. Species Crossreactivity: Xenopus laevis, Xenopus borealis, XLKE-A6 cells; negative with Pleurodelis, Triturus, Rana, chicken, rat, mouse. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
External Links
|
