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You are here:Home » Antibodies » Abs to Nuclear Proteins » Anti -Nucleolin (NCL, Nucl, C23, FLJ45706, Protein C23)

Anti -Nucleolin (NCL, Nucl, C23, FLJ45706, Protein C23)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP
Nucleolin is an abundant, 106kD nucleolar phosphoprotein. It is the major protein in actively dividing cells, whereas the degraded forms are relatively abundant in non-dividing cells. Stability of the nucleolin protein is cell proliferation-dependent. It plays a role in rDNA transcription, organization, and rRNA processing. It may alter DNA organization in response to cell cycle controls. It is also a good mammalian nucleolus marker.
Catalog #N6945-01C
ApplicationsSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in TBS, pH 7.2, 0.1% BSA, 0.1% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenThe epitope recognized maps to a region between residue 250 and 300 of human Nucleolin using the numbering given in entry NP_005372.2 (GeneID 4691).
SpecificitySpecies Crossreactivity: Human. Initial blots performed on lysate from NIH3T3 cells failed to provide evidence that this antibody reacts with the mouse ortholog. Other species have not been tested.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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