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Anti -Nucleolin (NCL, Nucl, C23, FLJ45706, Protein C23)
Anti -Nucleolin (NCL, Nucl, C23, FLJ45706, Protein C23)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Serum |
B IH IF |
|
| Nucleolin is an abundant, 106kD nucleolar phosphoprotein. It is the major protein in actively dividing cells, whereas the degraded forms are relatively abundant in non-dividing cells. Stability of the nucleolin protein is cell proliferation-dependent. It plays a role in rDNA transcription, organization, and rRNA processing. It may alter DNA organization in response to cell cycle controls. It is also a good mammalian nucleolus marker. | | | Catalog # | N6945-01E | | Cellular Localization | Nucleoli | | Positive Control | PD31 murine pre-B cells for immunofluorescence and crude PD31 nuclear extracts for Western Blot. | | Applications | Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry (paraffin-embedded sections). Other applications not tested. | | Recommended Dilution | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50%glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Host | Rabbit | | Source | Human | | Concentration | As reported | | Form | As reported | | Purity | Whole antisera | | Immunogen | A fusion protein containing amino acids 284-709 of human nucleolin. | | Specificity | This antibody is specific for nucleolin protein. Species Crossreactivity: Human and mouse. Other species have not been tested. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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