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You are here:Home » Antibodies » Abs to Nuclear Proteins » Anti -Nucleolin (NCL, Nucl, C23, FLJ45706, Protein C23)

Anti -Nucleolin (NCL, Nucl, C23, FLJ45706, Protein C23)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum B IH IF
Nucleolin is an abundant, 106kD nucleolar phosphoprotein. It is the major protein in actively dividing cells, whereas the degraded forms are relatively abundant in non-dividing cells. Stability of the nucleolin protein is cell proliferation-dependent. It plays a role in rDNA transcription, organization, and rRNA processing. It may alter DNA organization in response to cell cycle controls. It is also a good mammalian nucleolus marker.
Catalog #N6945-01E
Cellular Localization Nucleoli
Positive Control PD31 murine pre-B cells for immunofluorescence and crude PD31 nuclear extracts for Western Blot.
ApplicationsSuitable for use in Immunofluorescence, Western Blot and Immunohistochemistry (paraffin-embedded sections). Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50%glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
ConcentrationNot determined
FormAs reported
PurityWhole antisera
ImmunogenA fusion protein containing amino acids 284-709 of human nucleolin.
SpecificityThis antibody is specific for nucleolin protein. Species Crossreactivity: Human and mouse. Other species have not been tested.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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