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You are here:Home » Antibodies » Abs to Nuclear Proteins » Anti -Nucleophosmin (NPM, B23, Numatrin, Anaplastic Lymphoma Kinase, p80, ALK, NPM-ALK)

Anti -Nucleophosmin (NPM, B23, Numatrin, Anaplastic Lymphoma Kinase, p80, ALK, NPM-ALK)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH
Nucleophosmin (NPM, B23, Numatrin) is a phosphoprotein associated with cell nucleoli. Treatment of cells with cytotoxic drugs may make nucleophosmin enter nucleoplasm. It is reported that nucleophosmin shuttles continuously between cytoplasm and nucleoli to carry newly synthesized ribosomal proteins into nucleoli. NPMALK is a hybrid of the anaplastic lymphoma kinase (ALK) gene and the nucleophosmin (NPM) gene resulting from the t(2;5)(p23;q35) translocation found in anaplastic large cell lymphoma. In acute promyelocytic leukemia NPM gene is fused to RARalpha gene in (5;17) (q33;q12) transition.
Catalog #N7100-15
ApplicationsSuitable for use in Immunohistochemistry. Other applications have not been tested.
Recommended DilutionImmunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 min at RT.
Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by the researcher.
Positive ControlTonsil
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol 40-50%, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG/k
Clone No3F291
HostMouse
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See N7100-15X
PurityPurified by Protein G affinity chromatography.
ImmunogenGST fusion protein containing the N-terminal part of nucleophosmin fused to 14 aa of ALK protein.1.
MW of Antigen38kD. Epitope: N-terminal.
Cellular LocalizationNuclear
SpecificityRecognizes human Nucleophosmin (NPM, B23, Numatrin).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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